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Cell and Tissue Culture
A manual for laboratory workers, for years in its various editions, the authoritative handbook of practical tissue culture techniques (Freshney), cited in nearly 400 publications; "No other text on tissue culture has so wide or such well balanced coverage" (Am. Sc. Review). Offered is an historic specimen, originally owned by Elsa Meyer Zitcer, who without even a B.S., became a remarkable technician and made important contributions to the development of culture techniques for primary cells and cell lines including the first demonstration of growing poliovirus in primary cells from human amnion (1955). Paul and Zitcer's association is documented by his preface acknowledgment to her, vi, lines 2-7. Zitcer signed the endpaper and preserved the original letter Paul sent her Feb. 7, 1957, an invitation-she accepted-to join staff for the Tissue Culture Association Summer Course, Denver: the courses supplied most material for the book. Letter excerpts: "The job would involve handling serial cultures of L cells and HeLa for virology teaching." "It is an excellent opportunity to meet a large number of the outstanding figures in the tissue culture world." "I hope you will be interested, we should be glad to have the benefit of your green thumb." TLS: a typed page, folded blue airmail format, body of 26 lines, plus headings and salutations, with carefully done signature by Paul, sender and recipient addresses on reverse side. PUBLISHING NOTE: made up from British sheets with American publisher's boards, sheets printed from type by Central Press, Aberdeen and stating in gilt letters on upper cover side, Williams & Wilkins, Baltimore. (The expanded second edition was published in 1960. There have been five editions.) LETTER: laid-in. FURTHER ADDITION: laid-in print-out cutting with a Healy and R. C. Parker serial medium formula from an unpublished experiment. Laid-in material is now in an archival sleeve. PAUL [1922-1994]: Noted for his chromatin function research. Director H.E.R.T. Tissue Culture Laboratory, Glasgow University (1953-66); Titular Professor of Biochemistry, Glasgow University (1964-66); Director Cancer Research Laboratories, Royal Beatson Memorial (1966-70); Founding Director, Beatson Institute for Cancer Research (1970-87). ZITCER [1913-97]: Chief Technician, Virus Lab, University of California, Berkeley (ca. 1951-71), Lab Director-Wendell Meredith Stanley. In 1955 Zitcer with associates demonstrated it was possible to grow generations of human amniotic cells in serial culture and infect them with poliovirus. "Monolayer cultures of trypsin-dispersed cells derived from human amnion have been used for the propagation of mammalian viruses since 1955" (Grogan). The process would later be adapted to develop influenza vaccines. Primary culture amnion cells have a limited growth capability in vitro. After a few months subcultures become difficult to establish. Cells of established lines cultured from human amnion have far more growth potential but display an increased mitotic index including hyperchromatic nuclei, making them cells with malignant characteristics. The malignant features of transformed amnion cells in contrast to primary culture amnion cells made possible model in vitro experiments, demonstrated by Zitcer and Dunnabecke in 1957, valuable for study of cancerous cell growth, etc. Zitcer was the primary or co-author for ten significant papers on aspects of cell culture with applications to virus and cancer research (1954-1968). Papers include: Serial cultivation of normal human embryonic cells directly on glass (1954), Effect of serum ultrafiltrate on cultivated mast cells and fibroblasts from human skin (1954), Human amnion cells for large-scale production of polio virus (1955), Transformation of cells from normal human amnion into established strains (1957), Preparation of cell suspension from insect tissues for in vitro cultivation (1958), Multiplication of an influenza virus strain in a continuous line of mammalian cells (1967). CONDITION: Letter: fair, clean with many slight fingering marks, irregular tear-off of bottom corner -no text affected, pinholes at top inner corner. Cutting: very good. Book Condition Detail: Pages: largely clean: bottom margin spot on pp. 35-38 with trace offset to p. 34 and pp. 39-40: some bibliography entries with pencil checks by Zitcer. Boards: light soiling: light edgewear: slight spine wear at foot: bottom corner bumped. Gilt: few letters slightly dulled. Binding: excellent. Jacket: lacking. FORMAT: Publisher's sewn black cloth boards, gilt letters on upper side and spine. SIZE: Octavo, 221 x 149 mm (8.7 x 5.87 inches). PAGINATION: Confirmed complete, 269 pages, illustrated with 9 captioned plates on coated paper, 3 in color, 41 b/w figures and 16 tables: (iv), v- viii; 1-261 pages with subject index. Each of the 19 chapters ends with a separate, selected bibliography. BACKGROUND: Primary cell cultures (before subculturing) are used to grow viruses. Most are derived from primate kidney tissue, amnion, or fibroblasts from chick embryos. 1936; Sabin and Olitsky at Rockefeller Institute grow poliovirus in culture of brain tissue from a human embryo. 1949; John Franklin Enders and team culture poliovirus in non-nervous tissue. 1949; Finders and team grow poliovirus in rhesus monkey kidney tissue. 1951; Mary Kubicek, under Dr. George Gey at Johns Hopkins uses roller-tube technique to grow first human cancer transformed cell line, (from cervical carcinoma biopsy taken from Henrietta Lacks), cell line named HeLa. 1955; Zitcer, culture of poliovirus from human amnion. CATALOGING: John Paul is often confused with John Rodman Paul, a distinguished scientist associated with polio research at Yale. REFERENCE: Freshney, Culture of Animal Cells; Fraenkel-Conrat, Viruses; Corbis Archive, Zitcer portrait photo. ADDITIONAL IMAGES: by request.
      [Bookseller: Steven Waldman]
Last Found On: 2013-07-20           Check availability:      Biblio    

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